Genetic Stability of In Vitro raised Sugarcane Plantlets by RAPD Markers

Srivastava, Sangeeta and Jain, Radha and Gupta, P. S. and Singh, Jyotsna (2006) Genetic Stability of In Vitro raised Sugarcane Plantlets by RAPD Markers. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 7 (1-2). pp. 93-96.

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Abstract

Micropropagated plantlets produced by shoot-tip culture of two varieties of sugarcane were evaluated for genetic stability using randomly amplified polymorphic DNA (RAPD) analysis. Distinct and reproducible RAPD markers obtained through eight decamer random primers gave almost monomorphic banding profile for both the genotypes except a few weak bands across all the plantlets analyzed. Thus, a total of 82 bands were generated, exhibiting homogeneous RAPD patterns for the plantlets tested. These results suggest that the in vitro culture conditions used for shoot-tip culture are appropriate for clonal propagation of sugarcane, as they do not seem to interfere with the genetic integrity of the regenerated plantlets.

Item Type: Article
Subjects: Oalibrary Press > Biological Science
Depositing User: Managing Editor
Date Deposited: 09 Dec 2023 03:56
Last Modified: 09 Dec 2023 03:56
URI: http://asian.go4publish.com/id/eprint/3430

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