PRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY Aspergillus niger FROM BROWN MID RIB Sorghum VARIETIES

The present study reported with the production of enzyme cellulases (CMCase, Fpase and β-glucosidase) and xylanase by submerged fermentation using Aspergillus niger and sorghum hybrid non-BMR CSH 22 variety & low lignin BMR 22 and 24 substrates were evaluated after pre-treating with H2SO4 & NaOH. The highest cellulose and low lignin reported pretreatment conditions were applied for substrates and used the same in the fermentation. Maximum CMCase, FPase, and β-glucosidase production was observed for pretreated BMR 22 Sorghum biomass with values 30.5 ± 3.75 IU/ml, 2.74±0.34 IU/ml, and 9.6 ± 0.37 IU/ml respectively. But maximum xylanase production 15.8±1.75 IU/ml was observed while using untreated CSH 22 sorghum biomass as substrate. Results indicate low lignin mutant BMR22 and 24 substrates were promising substrates for the production of cellulase enzymes.