SUCCESSFUL TRANSFECTION OF LYMPHOBLASTOID CELL LINE USING MOLECULAR TECHNIQUE

The Lymphoblastoid cell line (LCL) is the immortal cell line that is produced after infecting the B lymphocytes with Epstein barvirus.This kind of cell is known to be hard for transfection practically.

In this, paperI am describing the protocols followed in my lab starting from infecting the B-lymphocytes with Epstein Barr virus (EBV) ending with successful transfection of LCL witharetroviral vector containinglac Z insert.

Materials and Methods: The importance of this work is that it describes away to preparea representative model for EBV-associated lymphoma through preparing LCLs then retrovirally infecting these cells with aretroviral vector.

Results: The lymphoblastoid cells (LCL) were produced after infecting the B-lymphocytes with EBV and successfully transfected with retroviral supernatant containing LacZ insert.

Conclusion: This work resulted in production of successfully transfected LCLs through infecting the b-lymphocytes with EBV virus then transfecting these cells with retrovirus vector.