Simultaneous Estimation of Ofloxacin and Ornidazole from Pharmaceutical Dosage Form by Spectrophotometric Methods

Two simple, sensitive, accurate, precise, rapid and economical methods were developed for the
simultaneous estimation of Ofloxacin and Ornidazole from combined tablet dosage form. First method
is based simultaneous equations and second method is based on Q-analysis (absorbance ratio
method). Ofloxacin and Ornidazole show absorbance maxima at 293nm and 275nm in 0.1N HCI
respectively. The linearity was obtained in the concentration ranges of 2-14 μg/ml for Ofloxacin and 5-
35 μg/ml for Ornidazole with Regression Coefficient (R2) greater than 0.999. In the first method
concentration and subsequently amount of drug determined by using simultaneous equations and in
second method concentration and amount of drugs determined by using ratio of absorbance at isoabsorptive
point(which was found to be 284 nm) and at λmax of one of the drug. The results of
analysis have been validated statistically and by assay and recovery studies. Therefore the proposed
validated method can be successfully applied for routine quality control analysis and simultaneous
determination of Ofloxacin and Ornidazole in combined drug formulations.