STUDY ON EVALUATING EFFICACY OF MICROWAVE TISSUE PROCESSING AND XYLENE FREE HEMATOXYLIN AND EOSIN STAINING PROCEDURES

Any tissue must be properly preserved and treated in order to retain its structure and saturate it with the appropriate medium. This allows for the creation of thin slices that can be stained and examined under a microscope. But the legacy of the conventional method of histo-processing is being used today. Despite the complexity and length of these processes, the traditional approach is still popular. Xylene and graded alcohols are also used in the H and E staining process in addition to hematoxylin and eosin. The microwave that was employed in our investigation operates under the premise that electromagnetic fields cause molecules to be excited, which causes them to rotate. As a result, heat is generated internally in the materials. In contrast to traditional heating, this heat increases the rate of fluid diffusion in and out of the tissue blocks or sections considerably more efficiently. In order to determine whether microwave processing can produce good staining as effectively as conventional processing, as well as to determine the effectiveness of dishwashing solution (1.7%) as a deparaffinizing agent for traditional Eosin and Hematoxylin stained slides in place of xylene, a prospective randomised comparative clinical study was planned as part of this research project.