Response of T98G Glioblastoma Cells Line to Wortmannin and Platelet-derived Growth Factor

Platelet derived growth factor (PDGF) and wortmannin are two well-known factors that have been used to study the response of various types of cancer cells. Both are related to the expression and activity of phosphatidylinositol 3-kinase (PI3K), and its effect on these tumor cells. The phosphatidylinositol-3-kinase (PI3K) and the PDGF signaling pathways are both crucial to many aspects of cell growth and survival, in physiological as well as in pathological conditions. The research study aims to evaluate the effects and cellular response of T98G glioblastoma cells. shown that cells dosed with platelet-derived-growth-factor (PDGF) and /or wortmannin, an inhibitor of PI3 kinase, proliferated at expected rates with respect to cells deprived of any additions. Cells with added platelet-derived-growth-factor (PDGF) multiplied substantially faster than naturally growing cells-some thirty percent. As anticipated, cells given only wortmannin divided over forty percent slower than cells without any dosage. Additionally, cells transfected with a luciferase reporter vector carrying consensus sequences of the nuclear factor NF-KB and AP-1 demonstrated that wortmannin treatment partially blocked the activation and expression of the NF-KB luciferase, while the activation and expression of the AP-1 reporter vector was clearly increase in T98G glioblastoma cells. However, the effect of wortmannin on the expression of NF-KB reporter vector was not observed by PDFG treatment. Our data indicate that Wortmannin and PDGF play different role in the control and expression of Phosphoinositide 3-kinase and in the regulation of different nuclear factor.