Antimutagenicity and Anticancer Effects of Biebersteinia multifida DC

Dabaghian, Fataneh and Dabaghian, Fataneh Hashem and Entezari, Maliheh and Ghobadi, Ali and Hashemi, Mehrdad (2013) Antimutagenicity and Anticancer Effects of Biebersteinia multifida DC. Annual Research & Review in Biology, 4 (6). pp. 906-913. ISSN 2347565X

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Abstract

Aims: The purpose of this research is to examine antimutagenicity and anticancer effect of Biebersteinia multifida.
Study Design: Currently cancer is considered as one of the main factors of mortality globally. Many chemicals in our environment can cause genetic mutations and are potentially responsible for millions of cancer-related deaths. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence.
Place and Duration of Study: Biebersteinia multifida DC. dried roots were purchased from local market in Tehran bazar, the center of Tehran province, Iran, between June 2012 and January 2013.
Methodology: In this study human leukemia pre B-cells (Nalm-6) were cultured in RPMI 1640 [Sigma], supplemented with 10% fetal bovine serum (FBS), penicillin -streptomycin and L-glutamine. The cultures were incubated at 37ºC, 5% CO2 and then inhibitory effect of ethanolic extract on their proliferation was measured by MTT assay. The ethanolic extract was evaluated in terms of antimutagenicity properties by a standard reverse mutation assay (Ames Test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100).Thus, it requires histidine from a foreign supply to ensure its growth. The aforementioned strain gives rise to reverted colonies when expose to carcinogen substance (Sodium Azide).
Results: The ethanolic extract prevented the reverted mutations and the hindrance percent 51.2% in antimutagenicity test. During MTT, human leukemia pre B-cells revealed to have a meaningful cell death when compared with controls (P = .01).
Conclusion: This study demonstrates the antimutagenicity effect of Biebersteinia multifida DC, and suggests that it may be potentially usefull as anticancer agent.

Item Type: Article
Subjects: Oalibrary Press > Biological Science
Depositing User: Managing Editor
Date Deposited: 10 Oct 2023 05:33
Last Modified: 10 Oct 2023 05:33
URI: http://asian.go4publish.com/id/eprint/2629

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