In silico ATP Synthase Inhibition Activity and Antibacterial Activity of Selected Essential Oil against Escherichia coli and Resistant Acinetobacter baumannii

The rise in the resistant strains has ignited the investigation of the ability of these essential oils to have an effect against them. The aim of this study was to investigate the antibacterial activity of clove oil, oregano oil and tea tree oil against Escherichia coli and resistant patient isolate Acinetobacter baumannii. Ciprofloxacin was used as standard and DMSO at 1 % was the negative control. To understand the inhibitory action on ATP synthase, docking analysis was done by using Autodock ver. 4.2.6. The study found out that essential oils had potent antibacterial activity against both E. coli and A. baumannii as all the essential oils recorded zones above 15 mm against E. coli and above 20 mm against A. baumannii. Tea tree oil had the highest activity among the three essentials against E. coli with 21.000±0.00 mm and against A. baumannii 28.500±0.500 mm respectively. These results support the antibacterial activity of essential oils. Docking analysis confirmed the ability of active constituents of selected essential oils to inhibit ATP synthase enzyme which is a crucial drug target in antimicrobial activity. Natural products have been at the center stage in the research for alternative agents with antibacterial agents.