Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications

González, María Isabel and González-Arjona, Mario and Santos-Coquillat, Ana and Vaquero, Javier and Vázquez-Ogando, Elena and de Molina, Antonio and Peinado, Héctor and Desco, Manuel and Salinas, Beatriz (2021) Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications. Biomedicines, 9 (1). p. 81. ISSN 2227-9059

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Abstract

The vertiginous increase in the use of extracellular vesicles and especially exosomes for therapeutic applications highlights the necessity of advanced techniques for gaining a deeper knowledge of their pharmacological properties. Herein, we report a novel chemical approach for the robust attachment of commercial fluorescent dyes to the exosome surface with covalent binding. The applicability of the methodology was tested on milk and cancer cell-derived exosomes (from U87 and B16F10 cancer cells). We demonstrated that fluorescent labeling did not modify the original physicochemical properties of exosomes. We tested this nanoprobe in cell cultures and healthy mice to validate its use for in vitro and in vivo applications. We confirmed that these fluorescently labeled exosomes could be successfully visualized with optical imaging. View Full-Text

Item Type: Article
Subjects: Oalibrary Press > Medical Science
Depositing User: Managing Editor
Date Deposited: 19 Nov 2022 03:57
Last Modified: 26 Dec 2023 04:45
URI: http://asian.go4publish.com/id/eprint/254

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