Zhao, Ming and Li, Yiting and Ji, Zhiqiang and Dou, Xiuping and Zhang, Chaoling and Li, Xiaohan and Liu, Shufang and Zhao, Li and Liu, Na (2022) Simultaneous Determination of Four Tetracycline Antibiotics Residues in Chicken by Solid Phase Extraction Coupled with High Performance Liquid Chromatography. Journal of Food Research, 11 (4). pp. 23-33. ISSN 1927-0887
63377e2e633e2.pdf - Published Version
Download (702kB)
Abstract
Objective: To establish a simple and rapid method for simultaneous determination of Tetracycline, Oxytetracycline, Chlortetracycline and Doxycycline in chicken. Methods: After optimized sample pretreatment and chromatographic conditions, tetracycline antibiotics in spiked chicken were extracted by ultrasonic extraction with Na2EDTA-Mcllvaine buffer solution (pH=4.0), purified and enriched by HLB solid phase extraction column, separated by Shim-pack GIST C18 column (4.6×150 mm, 3 μm), methanol-acetonitrile-oxalic acid (1:2:7, V: V: V) as the mobile phase, the flow rate was 0.9 mL/min, the UV detection wavelength was 350 nm, and the peak area was quantified by external standard method. Results: The linearity of the four tetracycline antibiotics was good in the range of 0.05-1 mg/kg (r>0.997), and the detection limits were 2.53 μg/kg ~ 5.94 μg/kg according to the S/N ratio (S/N) of 3. The relative standard deviations (RSD, n=6) of precision were 1.51%-4.80%. The recoveries were from 76.81% to 96.34%. Conclusion: The method has good selectivity, high accuracy and reproducibility, and is suitable for simultaneous determination of four tetracycline antibiotics in chicken.
Item Type: | Article |
---|---|
Subjects: | Oalibrary Press > Agricultural and Food Science |
Depositing User: | Managing Editor |
Date Deposited: | 03 Jun 2023 04:17 |
Last Modified: | 09 Jan 2024 07:03 |
URI: | http://asian.go4publish.com/id/eprint/2219 |