GC-MS Analysis of Methanolic Extracts of Leaf and Stem of Marsilea minuta (Linn.)

Aims: To analyze the chemicals composition of methanolic extracts of leaf and stem of M. minuta collected from Uppur Village, Tamilnadu, India by GC-MS.

Study Design: Experimental study.

Methodology: The Methanolic extracts were prepared and concentrated at 40°C using hot air oven. The concentrated methanolic extracts were subjected to GC-MS analysis using the instrument Perkin Elmer Clarus 500.

Results: The methanolic extract of leaf of M. minuta showed the presence of 36 phyto compounds including n-Hexadecanoic acid (44.41%) (C16H32O2); (Z)6,(Z)9-Pentadecadien-1-ol (35.49%) (C15H28O); Phytol (5.10%) (C20H40O); 2-Cyclohexane-1-one,4-hydroxy-3,5,6-trimethyl-4 (3-oxo-1-butenyl) (2.25%) (C13H18O3); 9,12,15-Octadecatrienoic acid (Z,Z,Z) (2.0%) (C18H30O2); 3,7,11,15-Tetramethyl-2-hexadecan-1-ol (1.99%) (C20H40O) and Benzofuran, 2,3-dihydro- (1.1%) (C8H8O). The methanolic extract of stem of M. minuta showed the presence of 27 bioactive compounds including 1,19-Eicosadiene (40.94%) (C20H38); n-Hexadecanoic acid (20.13%) (C16H32O2); 2(R)3(S)-1,2,3,4-Butanetetrol (16.31%) (C4H10O4); Glycerin (6.70%) (C3H8O3); Octadecanoic acid (4.24%) (C18H36O2); Benzofuran, 2,3-dihydro- (1.73%) (C8H8O); 3,7,11,15 – Tetramethyl-2 hexane decen-1-ol (1.55%) (C20H40O); n-Hexadecanoic acid methyl ester (1.15%) (C17H34O2) and 10- Octadecenoic acid methyl ester (1.42%) (C19H36O2).

Conclusion: The results of the present study concluded that the presence of various phytocompounds in leaf and stem of M. minuta. Therefore, plants are the rich sources of chemicals but largely unknown and unexplored. So, it is our hope that this study will encourage further research on isolation and purification of therapeutically important phytocompounds from M. minuta